Journal: Clinical and Vaccine Immunology : CVI

Article Title: Immunoproteomic Identification of In Vivo -Produced Propionibacterium acnes Proteins in a Rabbit Biofilm Infection Model

doi: 10.1128/CVI.00760-14

Figure Lengend Snippet: Schematic flow chart of the implant-associated osteomyelitis model in rabbits. After a 5-day incubation of the P. acnes strain RMA 13884 with dextran beads, rabbits were infected with a P. acnes inoculum in the left tibia. After 4 weeks of infection, blood serum was isolated, and X rays of both tibias were performed. The tibias were macroscopically and histologically examined, and the bacterial loads were quantified and reported as CFU per g of bone.

Article Snippet: SEM studies confirmed biofilm formation in both P. acnes strains (ATCC 11827 and RMA 13884) incubated with soda-lime solid-glass beads for 2 days at 37°C under static conditions. shows a young P. acnes (ATCC 11827 strain) biofilm with embedded cells within an exopolymeric matrix that appears similar to the glycocalyx of the biofilms of staphylococci. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 Scanning electron micrograph (SEM) of the P. acnes strain ATCC 11827 biofilm on solid glass beads (Walter Stern, Inc., Port Washington, NY) incubated for 2 days anaerobically at 37°C under static conditions (magnification, 2,000× [left panel] and 20,000× [right panel]), using a Zeiss Supra 55VP field emission scanning electron microscope with a beam accelerating voltage of 1 kV and a working distance of 3 mm.

Techniques: Incubation, Infection, Isolation

Journal: Frontiers in Microbiology

Article Title: The transportome of the endophyte Serendipita indica in free life and symbiosis with Arabidopsis and its expression in moderate salinity

doi: 10.3389/fmicb.2023.1191255

Figure Lengend Snippet: Functional complementation of Na + transport-defective mutants of Saccharomyces cerevisiae with Serendipita indica SiNHA1 and SiNHX1 . A drop test assay was performed by placing 5 μl of 10-fold serial dilutions of suspensions of the mutant AXT3K transformed with SiNHA1 and SiNHX1 cDNAs cloned in the shuttle vector pYPGE15 on a solid medium. The growth of the transformants expressing the S. indica antiporters was compared with that of transformants expressing the fungal ENA ATPases SiENA1 and SiENA5 or the empty vector. The growth was studied in AP (A) or YPD (B) media (see Materials and methods) at different pH values and in the presence of varying concentrations of NaCl and KCl as indicated.

Article Snippet: Col-0 Arabidopsis plants and S. indica strain DSM 11827, DSMZ, Braunschweig, Germany, were used throughout this study.

Techniques: Functional Assay, Mutagenesis, Transformation Assay, Clone Assay, Plasmid Preparation, Expressing